Document Details

Document Type : Thesis 
Document Title :
MOLECULAR IDENTIFICATION OF ORAL CAVITY MICROBIOME IN PATIENTS WITH SICKLE CELL DISEASE AT KING ABDULAZIZ UNIVERSITY HOSPITAL
التعريف الجزيئي لبكتريا التجويف الفموي لدى مرضى فقر الدم المنجلي بمستشفى جامعة الملك عبدالعزيز بجدة
 
Subject : Faculty of Science 
Document Language : Arabic 
Abstract : Sickle cell anemia (SCA) is a genetic disorder that affects the red blood cells. It is predominantly found in the Middle East. SCA patients and are being considered as a public health issue as because it affects a significant percentage of the world’s population especially in the middle east and Africa. The aim of this study was to Molecularly identify the Oral Cavity Microbiome in Patients with SCA at King Abdulaziz University Hospital Jeddah – Saudi Arabia. It also aimed to study the association of dental caries among sickle cell disease patients in relation to their oral microflora. The study's objectives were also to uncover the composition of oral microbiota in a group of patients who are sickle cell anemic and to shed new lights about how the oral microbiota affects their health in relation to their general health, age, and sex. The oral microbiome is dependable on multiple multifactorial circumstances such as, environmental and the host general health, for example hereditary sicknesses such as SCA plays a has a huge rule on the influence of the oral microflora of the patient. In this research, the oral microbiomes of 18 SCA patients in King Abdulaziz University Hospital were analyzed with respect to the core oral microbiome, their age and sex. We collected 18 samples of saliva from patients male and female (age 11–49) living in Makkah province. The 18 saliva samples were subjected to bacterial DNA extraction and purification. The samples were then sent for Next generation sequencing (NGS) of the 16S-V3-V4 regions. From eighteen samples, 2,451,168 effective reads and over 407 operational taxonomic units (OTU) was generated at a 99.52%. At the phylum level, there was a clear dominance of bacteria belonging to the Firmicutes phylum. This was followed by Proteobacteria and Bacteroidetes, with 346 different bacterial species being detected from all the SCA patients. These three phyla are all well known to cause various oral complications, such as periodontitis and oral cavity infections, due to their acidity. The oral microflora of patient living in Makkah – Saudi Arabia area can also be affected by external ecological and cultural level influences. Additionally, the composition of the oral microflora can influence the risk of more complicated and severe health problems for patients with SCA. 
Supervisor : Dr. Faisal Al-Sarraj 
Thesis Type : Master Thesis 
Publishing Year : 1445 AH
2023 AD 
Added Date : Wednesday, December 27, 2023 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
محمد عبدالله العتيبيAlotaibi, Mohammed AbdullahResearcherMaster 

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