Document Details
|
Document Type |
: |
Thesis |
|
Document Title |
: |
Histological Studies on the Effect of Euphorbia Inarticulata Plant Extract On Liver and Kidney Tissues in Rats: In Vitro and In Vivo Study دراسات نسيجية على تأثير مستخلص نبات (Euphorbia inarticulata) على أنسجة كبد وكلى الجرذان: دراسة مختبرية وحيوية. |
|
Subject |
: |
Faculty of Science |
|
Document Language |
: |
Arabic |
|
Abstract |
: |
The existence of Euphorbia inarticulata Schweinf in Jazan region and lack of literature researches on it encourage the present study for 1- phytochemical screening by GC–MS of the active components of fractionated extractions, hexane, ethyl acetate and methanol plant extracts. 2- In vitro evaluation of their anticancer activity against three cell lines, breast carcinoma (MCF-7), prostate (PC-3), hepatocellular carcinoma (HEPG2), antioxidant and their antimicrobial potentials assessment. 3-In vivo evaluation of the methanol extract administration dosed 250 and/or 400 mg/kg in normal and hepatocellular carcinoma (HCC) rat model induced by DENA/CCl4 through biochemical and histopathological investigations. Results showed that lupeol and β-amyrin were the most abundant triterpinoids in all extracts and the most abundant in hexane and ethyl acetate were steroids while in methanol were fatty acids. In vitro, E. inarticulata methanol extract showed the highest activity against used cancer cell lines. As well as the highest in free radical scavenging activity. Moreover, the most effective against used microbes all as compared to hexane and ethyl estate extracts results. In vivo LD50 estimated for orally administered methanol extract in rats was 4 g/kg. Biochemically, serum's liver and kidney function markers and tumor markers altered in the rat HCC model group as compared to the control values through significant increased AST, ALT, ALP, LDH and -GT enzymes activities and urea, uric acid, creatinine, T-BIL, AFP, TNF- IL-6 and significant decreased albumin and adiponectin levels. Supplementation of E. inarticulata methanol extract by either 250 mg or 400 mg to the animal model showed significant reverse in the aforementioned enzymes activities and the other biochemical levels. The higher dose showed advantage over the lower one in normalizing the AST, LDH and -GT enzymes activities. The liver and kidney antioxidants parameters estimated showed in HCC group as compared to control values significant increase in liver and kidney MDA and NO contents and significant decrease in the tissues GSH content and SOD, GPx and GST enzymes activities. Both 250mg and 400mg rat model supplemented groups recorded significant reverse of the aforementioned tissues antioxidants as compared to the corresponding values of the rat model group. Histopathologically, HCC group showed severe bile duct hyperplasia, severe vacuolar degeneration in hepatocytes with nuclear Pyknosis, fibrosis in portal area, congested and dilated portal vein, hyperactivation and proliferation of kupffer cells, inflammatory cells aggregation, cytoplasm fatty changes, hemorrhage proliferation of connective tissue and necrosis of hepatocytes. While, liver section of 400 mg treated rats showed slight karyomegally of hepatocytic nuclei and foamy cytoplasm of hepatocytes and bi nucleation of hepatocytes. While, kidney cortex of HCC group revealed dissociation of glomerulus, perivascular oedema, inflammatory cells infiltration and congested hypertrophied blood vessel, focal mononuclear aggregation, fibrous connective tissue proliferation, necrosis and a nucleated epithelial cell in the renal tubules and intertubular hemorrhage. The supplemented model with either 250 or 400 mg/kg extracts detected normal cortical region with normal renal corpuscles and renal tubules except some renal tubules showed swelling of epithelial cells and normal medullary region. The Bax and Bcl-2 protein in liver and kidney immunostaining showed in HCC model group, Bax immunostaining in the cytoplasm of liver cells and glomeruli and renal tubules, while faint cytoplasmic reaction to Bcl-2 antibodies. The methanol extract supplemented groups showed protection effect in a dose dependent manner of the hepatorenal apoptosis in the HCC induced rat model by downregulation of the Bax and Bcl-2 protein expressions. |
|
Supervisor |
: |
Prof. Dr. Samar Omar Abdullah Rabah |
|
Thesis Type |
: |
Doctorate Thesis |
|
Publishing Year |
: |
1441 AH
2020 AD |
|
Added Date |
: |
Sunday, June 7, 2020 |
|
Researchers
| غالية حسين المالكي | Almalki, Ghaliah Hussain | Researcher | Doctorate | |
|
Back To Researches Page
|