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Document Type |
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Thesis |
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Document Title |
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Bioremediation of Crude Oil-Contaminated Effluents Using Bacterial Biofilters المعالجة الحيوية لمياه الصرف الملوثة بالزيوت البترولية باستخدام المرشحات الحيوية البكتيرية |
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Subject |
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Faculty of Science |
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Document Language |
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Arabic |
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Abstract |
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Wastewaters containing oil must be treated before discharge in water bodies to avoid environmental pollution and comply with environmental protection regulations. The objective of this study was to investigate the ability of some Pseudomonas and other bacterial spices. for biodegradation of pollutants included in an oily industrial wastewater. Wastewater quality parameters including pH, total dissolved solids (TDS), biological oxygen demand (BOD), chemical oxygen demand (COD), bacterial total valuable count (TVC) and oil content were determined before and after treatment and the removal efficiencies were calculated. Screening bioassay was carried out using six exogenous environmental bacterial strains for degradation of oily wastewater to select the most promising. Two strains namely Enterobacter cloacae 279-56 (R4) and Pseudomonas otitidis MCC10330 (R19) could successfully eliminate oil content, in addition to reasonable removal of the organic load (BOD & COD). Therefore, they were considered the most promising and were selected to carry out bioremediation assays. They were tested as free living individuals or mixture for the removal of highly toxic oily contaminated industrial wastewater, collected from final drainage of oil refinery effluents which located in Alexandria, Egypt, as well as other contaminants in a batch experiment. Results showed that the two promising bacterial candidates (R4 and R19) considered the most efficient for all the tested parameters. The highest removal efficiency (RE) resulted in a residual levels of 1940, 171, 131, and 84 mg/l for TDS, BOD, COD and Oil content respectively in the treated oily wastewater effluents. Such levels are not compiling with the maximum permissible limits (MPL) for safe discharge into open environments, except for TDS. The mixed culture of the two selected strains showed higher removal efficiencies towards the included pollutants compared to the individual cultures. Therefore, the mixed culture was investigated in continues mode after fixation as a biofilm to treat the oily wastewater at different flow rates. Results obtained using the proposed biofilm were much better than those of the free living culture and were very close to MPL levels for safe discharge. The highest RE of oil content recorded 94.64% (7.51 mg/l) which considered excellent removal especially with the very short time (after 1 running h only). Higher removals of other parameters were also achieved that could reach levels below their MPL if more running time is applied or multiple biofilm unit in sequence is used. Therefore, the proposed treatment using the highly active bacterial selection considered highly efficient, very promising, renewable and cheap biotechnology for the treatment of heavily contaminated industrial wastewaters with high oil content. |
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Supervisor |
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Dr. Nidal M. O. Zabermawi |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1440 AH
2019 AD |
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Added Date |
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Sunday, May 5, 2019 |
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Researchers
| أماني حميد الهيبي | Alyhaibi, Amani Hameed | Researcher | Master | |
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